Can be used twice daily, but if only used once a day, at night is best. After cleansing your face, apply Eye Contour Serum gently to area around your eyes - but not on the eyelids. Patting gently in towards the nose is the best motion, using the 4th fingertip as it is the 'softest' and therefore less damaging to the sensitive eye area. Leave 5 to 10 minutes before applying make-up.